Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6

Liu, Dejian; Xi, Lijun; Han, Danyang; Dou, Ke; Su, Shijing; Liu, Jianguo

doi:10.1007/s10529-019-02661-x

Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6

Liu, Dejian; Xi, Lijun^*; Han, Danyang; Dou, Ke; Su, Shijing; Liu, Jianguo^*

Biotechnology Letters, 2019, 41(4-5): 583-589.

DOI: 10.1007/s10529-019-02661-x

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Summary

ObjectiveIdentification of a heavy metal ion-stimulated nitrilase with broad-spectrum substrate specificity.ResultsA novel nitrilase, PaCNit, was identified from Pannonibacter carbonis Q4.6 and its enzymatic properties were investigated. The maximum activity of PaCNit was observed at 65 degrees C and pH 7.0. PaCNit showed broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles, and was tolerant to different organic solvents. Remarkably, PaCNit activity was highly stimulated by metal ions, particularly by Ag  and Hg2 .ConclusionPaCNit nitrilase has a broad range of substrate specificity and can be activated by heavy metal ions. This specific characteristic makes it have a great potential for industrial application.

Keywords

Nitrilase; Pannonibacter carbonis; Heavy metal ions

Institution

China University of Petroleum (Huadong)

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