Chromatin Accessibility Dynamics during Chemical Induction of Pluripotency

Shangtao Cao; Shengyong Yu; Dongwei Li; Jing Ye; Xuejie Yang; Chen Li; Xiaoshan Wang; Yuanbang Mai; Yue Qin; Jian Wu; Jiangping He; Chunhua Zhou; He Liu; Bentian Zhao; Xiaodong Shu; Chuman Wu; Ruiping Chen; Waiyee Chan; Guangjin Pan; Jiekai Chen; Jing Liu; Duanqing Pei

doi:10.1016/j.stem.2018.03.005

Chromatin Accessibility Dynamics during Chemical Induction of Pluripotency

Shangtao Cao^#; Shengyong Yu^#; Dongwei Li^#; Jing Ye; Xuejie Yang; Chen Li; Xiaoshan Wang; Yuanbang Mai; Yue Qin; Jian Wu; Jiangping He; Chunhua Zhou; He Liu; Bentian Zhao; Xiaodong Shu; Chuman Wu; Ruiping Chen; Waiyee Chan; Guangjin Pan; Jiekai Chen; Jing Liu^*; Duanqing Pei^*

Cell Stem Cell, 2018, 22(4): 529-542.

DOI: 10.1016/j.stem.2018.03.005

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Summary

Despite its exciting potential, chemical induction of pluripotency (CIP) efficiency remains low and the mechanisms are poorly understood. We report the development of an efficient two-step serum-and replating-free CIP protocol and the associated chromatin accessibility dynamics (CAD) by assay for transposase-accessible chromatin (ATAC)-seq. CIP reorganizes the somatic genome to an intermediate state that is resolved under 2iL condition by reclosing previously opened loci prior to pluripotency acquisition with gradual opening of loci enriched with motifs for the OCT/SOX/KLF families. Bromodeoxyuridine, a critical ingredient of CIP, is responsible for both closing and opening critical loci, at least in part by preventing the opening of loci enriched with motifs for the AP1 family and facilitating the opening of loci enriched with SOX/KLF/GATA motifs. These changes differ markedly from CAD observed during Yamanaka-factor-driven reprogramming. Our study provides insights into small-molecule-based reprogramming mechanisms and reorganization of nuclear architecture associated with cell-fate decisions.

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