Application of plasmonic nanoprobes in cell imaging [等离子体纳米探针在细胞成像中的应用]

作者：Liu W.; Xie X.; Yin M.; Chen N.^*

来源：Nuclear Techniques, 2020, 43(9): 090501.

DOI：10.11889/j.0253-3219.2020.hjs.43.090501

• Cell imaging
• Dark-field microscopy
• Plasmonic nanoprobes
• Single particle analysis

Noble metal nanomaterials possess localized surface plasmon resonance (LSPR) effect. They are widely utilized in the fields of nanophotonics, chemical sensing, targeted radiation therapy, and bioimaging due to their unique physical, chemical, and optical properties. By adjusting the composition, morphology and size of the plasmonic nanoparticles, probes with distinct light scattering properties can be prepared and then monitored by dark-field microscopy (DFM). Compared with the fluorescent probes, plasmonic nanoprobes have better sensitivity and stability, which is suitable for live-cell imaging and real-time tracking of a single probe. Here, the latest development and some examples of plasmonic nanoprobes for dark-field imaging are introduced. Application in the observation of cell membrane receptors, in situ detection of intracellular biomolecules, and intracellular tracking are summarized, and perspects of plasmonic nanoprobes in DFM cell imaging are provided.

• 单位
  中国科学院大学