科研之友
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摘要
Objective: To evaluate the effect of Heyan Kuntai Capsule(和颜坤泰胶囊, HYKT) on the ovarian function of aged mice and expressions of cohesion complexes in oocytes. Methods: Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method(n=5 per group), including the control group(saline), 17β-estradiol group [E2, 100 μg/(kg·d)], and low-, medium-, and highdose of HYKT groups [0.3, 0.9, 2.7 g/(kg·d), respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X(γH2AX) staining and Td T-mediated d UTP Nick-End Labeling(TUNEL) assay. The density of cohesin subunits, REC8 meiotic recombination protein(REC8), structural maintenance of chromosome(SMC) 1β and SMC3 in oocytes were evaluated by immunofluorescent staining. Results: After administration of E2 and high-dose of HYKT, the total number of follicles as well as the number of primordial and primary follicles were significantly increased(P<0.05). Anti-γH2AX staining and TUNEL assay demonstrated that high-dose of HYKT and E2 partly suppressed the apoptosis of follicles(P<0.05). Furthermore, it showed an increased trend in the levels of REC8 and SMC1β, after administration with E2 and HYKT, and no obvious change in the level of SMC3. Conclusion: HYKT could enhance the number of follicles, suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits(REC8 and SMC1β) in oocytes of aged mice, indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.
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