Survivin-ASODN靶向治疗XWLC-50细胞移植瘤裸鼠的实验研究(英文)

作者：Weiwei Wang Shaojia Wang Gaofeng Li Lei Li Ruibing Cheng Department of Thoracic Surgery The Third Affiliated Hospital of Kunming Medical University Lung Cancer Research Center of Yunnan Cancer Hospital Kunming China Department of Gynecological Oncology The Third Affiliated Hospital of Kunming Medical University Kunming China

来源：The Chinese-German Journal of Clinical Oncology, 2012, (06): 324-326.

• survivin
• antisense oligomucleotide
• XWLC-05
• animal experiment

Objective:The aim of this study was to study the inhibiting effect of survivin mRNA on transplanted XWLC-05 tumor on nude mice.Methods:We established XWLC-05 transplanted nude mice model.44 mice would be divided randomly into 4 groups:control group (blank),Lip group (simple liposome),survivin SODN group (transfected by sense oligonucleotide) and survivin ASODN group (transfected by antisense oligonucleotide).We would study general activities of nude mice in these 4 groups,measure the size of tumor and calculate the tumor inhabiting rate also.Pathological methods were applied in the analysis of the effect of different treatment on heart,kidney and liver of nude mice in these 4 groups.Results:Tumor grew slowly and size,weight of tumor was lower in survivin ASODN group when compared with that of others.Nude mice of survivin ASODN group showed lower growth index and tumor inhabiting rate was significantly higher than that of other groups (P < 0.05).Transplanted tumor on nude mice in control group (blank),Lip group,and survivin SODN group grew bigger as time passed and there was no significance among them (P > 0.05).We found a great deal of tumor cell necrosis in survivin ASODN group.No death of nude mice was observed in all 4 groups and we did not found obvious lesion in vital organs.Conclusion:Survivin ASDON could be used for the inhibitation of subcutaneously transplanted tumor in nude mice without obvious lesion in vital organs.