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摘要
Anaerobic ammonia oxidation (anammox) process has the problem of unstable treatment efficiency in treating domestic sewage, which seriously hinders its engineering application of sewage treatment. In view of the above problems, this paper proposed to use anammox-MBR (AX-MBR) coupling process to treat sewage, which included anoxic reactor, aerobic reactor and membrane module. During the startup of the experiment, the activated sludge of the sewage treatment plant was added, then the dissolved oxygen of the system was reduced, and the anaerobic ammonia oxidizing bacteria (AnAOB) was added. The results showed that the addition of AnAOB could effectively improve the NH4+-N removal rate of AX-MBR, and the average NH4+-N removal rate increased significantly from 68% to 87%. During the experiment, the temperature of the reactor was not controlled. The results indicated that the activity of AnAOB decreased sharply with decreasing temperature when the temperature was lower than 20 ℃. During the operation in low temperature environment, the way of reducing the influent load could be adopted to ensure the treatment rate. The research data of 15N stable isotope tracer method on the nitrogen removal contribution rate of anammox showed that the nitrogen removal of AX-MBR was mainly completed by the way dominated by anammox, and its nitrogen removal contribution rate could reach 65%. The 16S high throughput sequencing results showed that the AnAOB in the anoxic reactor was mainly Candidatus Kuenenia, and the abundance of denitrifying bacteria in the anoxic reactor and aerobic reactor was greater than that of ammonia-oxidizing bacteria, which indicated that the NO2--N in AX-MBR mainly formed from partial denitrification, which proved the existence of partial denitrification/anammox process.The results of this study can provide reference for the development of anammox process. ? 2023 Science Press.
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