Phenytoin-induced Toxicity in the Postnatal Developing Cerebellum of Wistar Rats, Effect of Calotropis procera on Histomorphometric Parameters Toxicidad Inducida por Fenito赤na en el Desarrollo Postnatal del Cerebelo de Ratas Wistar, Efecto de Calotropis procera sobre los Par芍metros Histomorfom谷tricos

作者：I O Imosemi; A A Osinubi

来源：International Journal of Morphology, 2011.

• Fenitoina
• Toxicidad
• Desarrollo del cerebelo
• Calotropis procera
• Phenytoin
• Toxicity
• Cerebellar development
• Calotropis procera

The role of methanolic leaf extracts of Calotropis procera in phenytoin-induced toxicity on histomorphometric variables in the postnatal developing cerebellum of Wistar rat was studied. Pregnant rats were treated orally with 50 mg/kg phenytoin in pre and post natal life and 300 mg/kg methanolic leaf extract of Calotropis procera 1 hour prior to phenytoin administration. 200 mg/kg vitamin C (standard antioxidant) was also administered orally 1 hour prior to phenytoin treatment. The control animals received water. Standard diet of rat pellets and water were provided ad libitum. At the end of the experiment, the offspring of days 1, 7, 14, 21, 28 and 50 post partum, five per group were sacrificed by cervical dislocation. The cerebellum of all groups were dissected out and processed for histomorphometric studies. The results showed in the developing cerebellum of phenytoin treated animals, a delayed cell maturation in the external granular layer, reduction of the molecular layer, astrocytic gliosis and loss of Purkinje cells on day 50 postpartum. Administration of extracts of Calotropis procera and vitamin C though reversed these changes when compared with the phenytoin treated group, but not significantly when compared with the control. In conclusion, supplementation with methanolic extracts of Calotropis procera reduced the rate at which phenytoin induced toxicity in the postnatal developing cerebellum of Wistar rat. Fue estudiado el rol de los extractos metan車licos de las hojas de Calotropis procera en la toxicidad inducida por fenito赤na sobre las variables histomorfom谷tricas en el desarrollo postnatal del cerebelo de ratas Wistar. Ratas pre adas fueron tratadas por v赤a oral con 50 mg/kg de fenito赤na durante la vida pre y post natal. Adem芍s, fue administrado, por v赤a oral, una hora antes del tratamiento con fenito赤na 300 mg/kg de extracto metan車lico de las hojas de Calotropis procera y 200 mg/kg de vitamina C (antioxidante est芍ndar). Los animales control recibieron agua. Una dieta est芍ndar de pellets para rata y agua se proporcionaron ad libitum. Al final del experimento, 5 cr赤as por grupo de 1, 7, 14, 21, 28 y 50 d赤as post parto, fueron sacrificadas por dislocaci車n cervical. El cerebelo de todos los animales de los diferentes grupos fueron disecados y procesados para el estudio histomorfom谷trico. Los resultados mostraron en el desarrollo del cerebelo de los animales tratados con fenito赤na un retraso en la maduraci車n de c谷lulas en la capa granular externa, reducci車n de la capa molecular, gliosis astrocitaria y p谷rdida de las c谷lulas de Purkinje en el d赤a