Lactobacilos e bifidobact谷rias nas fezes de crianˋas escolares de dois estratos socioeconˋmicos: moradores em uma favela e alunos de uma escola particular

作者:Mello; Ricardo M P de; Morais; Mauro B de; Tahan; Soraia; Melli; Ligia C F L; Rodrigues; Mirian S do Carmo; Mello; Carolina S; Scaletsky; Isabel C A
来源:Jornal de Pediatria, 2009, 85(4): 307-314.
DOI:10.1590/S0021-75572009000400007

摘要

objective: to determine the number of lactobacillus and bifidobacterium colonies in the feces of schoolchildren from two different socioeconomic levels. methods: we analyzed fecal samples of children aged 6 to 10 years without gastrointestinal symptoms or recent use of antimicrobials. the first group included 86 children living in a favela in the city of osasco, state of sˋo paulo, southeastern brazil. the second group included 36 children attending a private school in the same city. body mass index (bmi) was used to assess nutritional status according to the reference values of the national center for health statistics (nchs). specific anaerobic culture media were used for isolation of colonies for 48 and 72 hours at 37 ~c. the number of colonies was determined using the plate-counting method. results: the mean lactobacillus (1.125 x 109 colony-forming units, cfu/g) and bifidobacterium (1.675 x 109 cfu/g) counts in the private school group were higher (p -1.0 sd (n = 57): 0.350 x 109 and 0.420 x 109 cfu/g, respectively. conclusion: the microbiota of schoolchildren living in unfavorable environmental conditions shows lower numbers of fecal lactobacillus and bifidobacterium colonies, especially in children with lower bmi values.

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