流体剪切力下 CD44-HA 介导的 MDA-MB-231 细胞及 HL60 细胞的滚动黏附

作者：Zhang Ying; Fang Ying^*; Wu Jianhua; Lin Yu; Ma Linlin

来源：Journal of Medical Biomechanics, 2023, 38(2): 220-227.

DOI：10.16156/j.1004-7220.2023.02.003

• CD44
• cell adhesion
• fluid shear stress
• hyaluronic acid (HA)
• tumor cell

Objective To explore how hyaluronic acid (HA) in extracellular matrix regulates the adhesion of CD44+ tumor cells. Methods MDA-MB-231 cells or HL60 cells were perfused in a parallel plate chamber. The movement of cells over immobilized HA was observed and analyzed to obtain the characteristics of cell adhesion and rolling. Results The adhesion number of MDA-MB-231 cells on HA substrate was positively regulated by HA concentration, but not by HA molecular weight. Compared with physically adsorbed HA, immobilized HA by avidin-biotin could significantly improve the cell adhesion ratio. With the increase of shear stress in the range of 30-50 mPa, the rolling velocity of cells increased and the adhesion ratio decreased, but the tether lifetime of cells was not affected. In the same flow field, compared with MDA-MB-231 cells, HL60 cells with low expression of CD44 rolled more quickly on immobilized HA, with shorter tether lifetime and much lower adhesion ratio (<1. 5%). Conclusions Fluid shear stress might mediate the rolling velocity of MDA-MB-231 cells by regulating the CD44-HA association rate rather than their dissociation rate. The interaction between CD44 and HA is involved in the initial adhesion of HL60 cells, but it does not play a major role. This study will provide references for the design of anti-tumor drugs. ? 2023 Editorial Department of Journal of Shanghai Second Medical University.

• 单位
  华南理工大学