科研之友
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摘要
introduction: gastric cancer is one of the commonest cancers in our country being responsible for a high mortality rate. recently, the expression of p53 and amplification of c-erb-b2 gene have been described in gastric carcinoma. the relevance and biological significance of these findings are not established yet. objective: the authors investigated p53, c-erb-b2 immunohistochemical expression in 482 cases of gastric carcinomas. material and methods: tissue microarray (tma) blocks were designed using replicate samples of paraffin-embedded tissue from 482 gastric carcinomas. sections were stained with he, and antibodies to p53 and c-erb-b2. cases were considered p53 positive if nuclear staining was detected in %26gt; 10% of the tumor cells. cases were assessed c-erb-b2 positive if the entire circunference of the cell membrane was stained in %26gt; 10% of the tumor cells. results: overall expression of p53 and c-erb-b2 was observed in 30% and 12% of gastric carcinomas cases, respectively. relationship with intestinal type of gastric carcinomas and expression of c-erb-b2 was shown (p %26lt; 0.001), and expression of p53 was observed in tumors larger than 5cm (p = 0.003). there was not significant difference in survival curves of patients with gastric cancer in relation to the expression of these markers. conclusion: intestinal type of gastric carcinomas in our country are more frequently positive for c-erb-b2 than diffuse type. the expression of p53 is associated with tumor size. the tma is a valid and efficient way to study immunohistochemical markers, having a high correlation with the full section.
