Fototerapia causa danos ao DNA de leuc車citos mononucleares perif谷ricos em rec谷m-nascidos a termo

作者：Aycicek; Ali; Kocyigit; Abdurrahim; Erel; Ozcan; Senturk; Hakan

来源：Jornal de Pediatria, 2008.

DOI：10.1590/S0021-75572008000200009

• dna damage
• comet assay
• hyperbilirubinemia
• oxidative stress
• phototherapy.

objective: our aim was to determine whether endogenous mononuclear leukocyte dna strand is a target of phototherapy. methods: the study included 65 term infants aged between 3-10 days that had been exposed to intensive (n = 23) or conventional (n = 23) phototherapy for at least 48 hours due to neonatal jaundice, and a control group (n = 19). dna damage was assayed by single-cell alkaline gel electrophoresis (comet assay). plasma total antioxidant capacity and total oxidant status levels were also measured, and correlation between dna damage and oxidative stress was investigated. results: mean values of dna damage scores in both the intensive and conventional phototherapy groups were significantly higher than those in the control group (p 0.05). there were no significant correlations between dna damage scores and bilirubin, total oxidant status and oxidative stress levels in either phototherapy group (p %26gt; 0.05). conclusions: both conventional phototherapy and intensive phototherapy cause endogenous mononuclear leukocyte dna damage in jaundiced term infants.