科研之友
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摘要
Reverse transcription-polymerase chain reaction(RT-PCR)was used to amplify the Small Envelope(E)gene of avian Infectious bronchitis virus(IBV)LX4 strain and the gene was cloned into the pMD18-T vector.By digestion with restriction enzymes SalⅠand BamHⅠ,the E gene was subcloned into pET-30a vector to...
