Altered Kidney Morphology and Enzymes in Streptozotocin Induced Diabetic Rats Morfolog赤a y Enzimas Renales Alteradas en Ratas con Diabetes Inducida por Estreptozotocin

作者：Muhammad Zafar; Syed Naeem ul Hassan Naqvi; Masood Ahmed; Zahid Ali Kaimkhani

来源：International Journal of Morphology, 2009.

• Estreptozotocin
• Aminotransferasas
• Fosfatasa alcalina
• Pseudocolinesterasa
• STZ-diabetes
• Ratas
• Streptozotocin
• Aminotransferases
• Alkaline phosphatase
• Pseudocholinesterase
• STZ-diabetes
• Rats

We studied the effects of streptozotocin (STZ)-induced diabetes on kidney morphology, anatomy, architecture and on the activities of aminotransferases (AST and ALT), alkaline phosphatase (ALP) and pseudocholinesterase (PChE) in albino rats. The aim of the study was to investigate the association between diabetic kidney complications and kidney enzyme alterations. This study was performed in the Department of Anatomy and Institute of Pharmaceutical Sciences, Baqai Medical University, Karachi and Pathology department of College of Physicians %26 Surgeons (CPSP) Pakistan in 2007-08. Diabetes was induced by a single dose of STZ (45 mg/kg, b.w.) given intraperitoneally in sodium citrate buffer at pH 4.5. Eighty (80) albino rats were divided into five groups: control (A) and STZ treated (B, C, D, and E) which were sacrificed 2, 4, 6 and 8 weeks post treatment respectively. Histopathology of kidney showed lesions similar to human glomerulosclerosis, glomerular membrane thickening, arteriolar hyalinization and tubular necrosis. Increased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and pseudocholinesterase (PChE) were observed in the kidney. It seems that the diabetic complications in the kidney are likely to be associated with alterations in enzyme levels. Se estudiaron los efectos de la diabetes inducida por estreptozotocin (STZ) sobre la morfolog赤a, anatom赤a, arquitectura y sobre las actividades de aminotransferasas (ALT y AST), fosfatasa alcalina (ALP) y pseudocolinesterasa (PChE) en los ri ones de ratas albinas. El objetivo del estudio fue investigar la asociaci車n entre las complicaciones renales diab谷ticas y la alteraci車n de las enzimas renales. Este estudio se realiz車 en el Departamento de Anatom赤a y el Instituto de Ciencias Farmac谷uticas, Universidad de Medicina Baqai, Karachi y el departamento de Patolog赤a de Colegio de M谷dicos y Cirujanos (CPSP) Pakist芍n entre el 2007-2008. La diabetes fue inducida por una dosis 迆nica de STZ (45 mg / kg de peso corporal) administrada por v赤a intraperitoneal en tamp車n de citrato de sodio a pH 4.5. Ochenta (80) ratas albinas fueron divididas en cinco grupos: control (A) y STZ tratados (B, C, D y E), que se sacrificaron a las 2, 4, 6 y 8 semanas despu谷s del tratamiento, respectivamente. La histopatolog赤a del ri 車n mostr車 lesiones similares a la glomeruloesclerosis en humanos, engrosamiento de la membrana glomerular, hialinizaci車n arteriolar y necrosis tubular. Aumento de los niveles de aspartato aminotransferasa (AST), alanina aminotransferasa (ALT), fosfatasa alcal