First UHPLC–MS/MS method coupled with automated online SPE for quantification both of tacrolimus and everolimus in peripheral blood mononuclear cells and its application on samples from co‐treated pediatric patients.

作者：Debora Pensi; Amedeo De Nicolò; Michele Pinon; Clarissa Pisciotta; Pier Luigi Calvo; Antonello Nonnato; Renato Romagnoli; Francesco Tandoi; Giovanni Di Perri; Antonio D'Avolio

来源：Journal of Mass Spectrometry, 2017, 52(3): 187-195.

DOI：10.1002/jms.3909

• tandem mass
• PBMCs
• intracellular
• everolimus
• tacrolimus
• OSM

Tacrolimus (TAC, FK‐506) and everolimus (EVE, RAD001) are immunosuppressors used to treat pediatric patients undergoing liver transplantation. Their hematic TDM by liquid chromatography became standard practice. However, it does not always reflect concentrations at their active site. Our aim was to ...