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摘要
OBJECTIVE To establish a high performance liquid chromatography-mass spectrometry (HPLC-MS) method for the quantitative determination of teicoplanin in human plasma. METHODS Plasma of 50 [xL was prepared by protein precipitation with acetonitrile, used daltomycin as internal standard (IS). Chromatographic separation was performed on a Welch Ultimate XB Ci8 column (2. 1 mm X 50 mm, 3 fxva) ; column temperature: 40 °C; mobile phase: acetonitrile-water (0. 5% formic acid) , gradient elution within 5. 5 min. The concentration of teicoplanin in plasma was determined by ESI ion source and positive ion mode multi-reaction monitoring method (MRM). The ion transitions were performed at ml z 940. 5-?-316. 2 for teicoplanin and mlz 811. 0-?-313. 0 for daltomycin, with the collision voltage of 20 eV and 42 eV, respectively. The specificity, carryover, matrix effect, linearity, accuracy and precision of the method, and the stability of plasma samples were investigated. RESULTS The linear relationship of plasma teicoplanin was good in the range of 1. 0~100. 0 mg'L-1 , and the lower limit of quantification was 1. 0 mg'L-1. The standard deviation (USD) of intra-batch and inter-batch precision was ^10. 9%, and the normalized matrix effect of teicoplanin was 92. 7%-~109. 3% and the RSD was ^12. 0%. Plasma samples may be stored frozen at - 20 °C for at least 41 days. No interference was found in clinical samples with good method stability. CONCLUSION The method is simple, sensitive, accurate and reliable, and is suitable for the determination of teicoplanin in human plasma. ? 2020 Editorial Office of Chinese Journal of Hospital Pharmacy.
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