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摘要
Biodegradation of 2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane (DDT) in soil by laccase extract from white rot fungi under different experimental conditions was investigated. DDTs, which stands for the sum of p,p'-DDE, o,p'-DDT, p,p'-DDD and p,p'-DDT in soil was degraded efficiently, and the residue decreased rapidly during the first 15 days and then slowly during the period of 16 25 days. The biodegradation of DDTs in soil fitted the pseudo-first-order kinetics. For 5, 10, 15 and 25 days of incubation with laccase, the residue of DDTs in soil under different atmospheres was decreased by 20%-33%, 34% -52%, 41%-61% and 41%- 69% respectively, under different flooding conditions that was decreased by 12%-17%, 17%-30%, 30%-45% and 35%-52% respectively,and for different soils that was decreased by 25% 34%, 39%-53%, 44%-58% and 47%-62% respectively. The half-life of DDTs in soil ranged from 15.07 to 32.95 days under O2, air or N2 atmospheres, 23.07 to 40.71 days under different flooding conditions, and 18.78 to 28.88 days for different soils. Laccase is an efficient and safe agent for bioremediation of DDT-contaminated soil.
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