Effects of Interferon-Tau (IFN-而) and Progesterone on Transcription Level of Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) in Cultured Bovine Endometrial Cells

作者：Jia Yao; Ming Zhang; Yu Mei Feng; Si Yi Chen; Wei Wang; Jie Wang; Song Jia Lai

来源：Journal of Animal and Veterinary Advances, 2012.

DOI：10.3923/javaa.2011.1833.1840

Interferon-tau (IFN-而) secreted by blastular trophectoderm was thought to be the primary signal for pregnancy recognition during bovine early pregnancy period after pregnancy recognition, progesterone dominated pregnancy maintaining. Moreover, Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) was important for embryo implantation. The objectives of studies were evaluated whether IFN-而 and progesterone regulated MMP-2 and TIMP-2 expression in cultured bovine Endometrial Cells (bECs). In the studies, bECs were cultured in DMEM-Ham s F12 medium with free-serum. These cultured cells were divided into 4 groups and cultured in four types culture medium: culture medium without IFN-而 and progesterone (Co group); culture medium including 100 ng mL-1 IFN-而 (IN group); culture medium including 200 nmol L-1 progesterone (Pr group); culture medium including 100 ng mL-1 IFN-而 and 200 nmol L-1 progesterone (I+P group). Cultured bECs were harvested and extracted total mRNA after being cultured for 1, 3, 6 and 12 h and then expression level of MMP-2 and TIMP-2 were assessed with fluorescence quantitative PCR. The results showed that MMP-2 expression level in Pr, IN and I+P group were extremely significantly higher than in Co group (p0.05); TIMP-2 expression level in Pr, IN and I+P group was extremely significantly lower than in Co group (p0.05). The conclusion indicated progesterone and IFN-而 induced MMP-2 expression in vitro cultured bECs whereas, progesterone and IFN-而 suppressed TIMP-2 expression in vitro cultured bECs.