Effect of Ethanol Extract of Whole Plant of Trichosanthes cucumerina var. cucumerina L. on Gonadotropins, Ovarian Follicular Kinetics and Estrous Cycle for Screening of Antifertility Activity in Albino Rats Efectos del Etanol Extra赤do de la Planta Completa de Trichosanthes cucumerina var. cucumerina L. sobre Gonadotropinas, Cin谷tica Folicular Ov芍rica y Ciclo para Screening de Actividad Antifertilidad en Ratas Albinas

作者：Devendra N Kage; Vijaykumar B Malashetty; Y N Seetharam; P Suresh

来源：International Journal of Morphology, 2009.

• Trichosanthes cucumerina var. cucumerina
• Antifertilidad
• Foliculogenesis
• Ciclo estral
• Gonadotrofinas
• Actividad 3 b-HSD
• 17 b-HSD
• Esteroidogenesis ovarica
• Trichosanthes cucumerina L. var. cucumerina
• Antifertility
• Folliculogenesis
• Estrous cycle
• Gonadotropins
• 3 b-HSD
• 17 b-HSD activity
• Ovarian steroidogenesis

Ethanol extract of whole plant of Trichosanthes cucumerina L. var. cucumerina was evaluated for antiovulatory activity in adult rats. The ethanol extract at the doses 200 and 400mg/kg body weight (orally) affected the normal estrous cycle showing a significant increase in estrus and metestrus phases and decrease in diestras and proestrus phases. The extract also significantly reduced the number of healthy follicles (Class I-Class VI) and corpora lutea and increased the number of regressing follicles (Stage IA, Stage IB, Stage IIA, and Stage IIB). The protein and glycogen content in the ovaries were significantly reduced in treated rats. The cholesterol level was significantly increased, whereas, the enzyme activities like 3b-HSD and 17b-HSD were significantly inhibited in the ovary of treated rats. Serum FSH and LH levels were significantly reduced in the treated groups were measured by RIA. In acute toxicity test, neither mortality nor change in the behavior or any other physiological activities in mice were observed in the treated groups. In chronic toxicity studies, no mortality was recorded and there were no significant differences in the body and organ weights were observed between controls and treated rats. Hematological analysis showed no significant differences in any of the parameters examined (RBC, WBC count and Hemoglobin estimation). These observations showed the antiovulatory activity of ethanol extract of whole plant of Trichosanthes cucumerina L. var. cucumerina in female albino rats. El extracto de etanol de toda la planta de Trichosanthes cucumerina var. cucumerina (L.) se evalu車 en cuanto a su actividad antiovulatoria en ratas adultas. El extracto de etanol en dosis de 200 y 400mg/kg de peso corporal (oral) afect車 el ciclo normal estral, mostrando un aument車 significativo en las fases de estro y metaestro y la disminuci車n de las fases de diestro y proestro. El extracto tambi谷n redujo significativamente el n迆mero de fol赤culos sanos (Clase I=Clase VI) y cuerpo l迆teo y aument車 el n迆mero de fol赤culos en regresi車n (etapa I, etapa IB, etapa II y etapa IIB). La prote赤na y el contenido de gluc車geno en los ovarios se redujeron significativamente en las ratas tratadas. El nivel de colesterol aument車 significativamente, mientras que, actividad de las enzimas 3b-HSD y 17b-HSD se inhibi車 significativamente en el ovario de ratas tratadas. FSH s谷rico y los niveles de LH se redujeron significativamente en los grupos tratados y medidos por R赤A. En la prueba de toxicidad aguda, no hubo mortalidad ni cambio en el comportamiento fisiol車gico o de cualquier