纳米电极-膜片钳监测单个活囊泡连续释放

作者：吴文展; 黄卫华; 王玮; 王宗礼; 程介克; 张蓉颖; 陈宇; 刘杰; 郑从义; 沈超

来源：高等学校化学学报, 2005, (05): 829-831.

DOI：10.3321/j.issn:0251-0790.2005.05.033

• 纳米电极
• 膜片钳
• 单囊泡空间分辨
• 连续囊泡释放

Carbon fiber nanoelectrode(tip diameter ca. 100 nm)-patch clamp was firstly applied to real-time monitoring dopamine release from single living vesicles of single Rat pheochromocytoma(PC12) cells with highly temporal and spatial resolution. It is first demonstrated that there are continual vesicle releases of dopamine at the same site in the active release zone, which play a main role in the exocytosis.

• 单位
  化学与分子科学学院; 华中科技大学; 武汉大学