The Effect of Xuefu Zhuyu Decoction(血府逐瘀汤) on in vitro Endothelial Progenitor Cell Tube Formation

作者：高冬; 吴立娅; 焦雨欢; 陈文元; 陈岩; Ted J Kaptchuk; 逯波; 宋军; 陈可冀

来源：Chinese Journal of Integrative Medicine, 2010, (01): 50-53.

• Xuefu Zhuyu Decoction
• endothelial progenitor cells
• tube formation

<正>Objective:To observe the effect of Xuefu Zhuyu Decoction(血府逐瘀汤)-containing serum (XFZYD-CS) on endothelial progenitor cell(EPC) tube formation in vitro.Methods:Mononuclear cells from rat bone marrow were prepared in a Ficoll density gradient centrifuge.EPCs were separated by the differential attachment method,and observed with inverted microscope for the effect of XFZYD-CS on EPC tube formation. Results:After one day,EPCs exposed to the serum containing 5%,10%and 15%XFZYD-CS formed typical tubes or vessel networks.The tube formation time was two days ahead of the control group and the size of most tubes in the serum groups was smaller than in the control group.Conclusion:XFZYD-CS could induce EPC angiogenesis and hasten tube formation,especially in capillary vessels.The study provides experimental evidence for the plausibility of Xuefu Zhuyu Decoction in the treatment of ischemic diseases.

• 单位
  Harvard Medical School; 中医学科学院