STAT-1分子在干扰素治疗慢性乙型肝炎不同应答者肝组织中的表达

作者：王鲁文; 龚作炯

来源：中华临床感染病杂志, 2008, (02): 79.

• Janus激酶
• 信号传导
• 转录激活因子
• 肝炎,乙型,慢性
• 干扰素

目的 探讨ifnα抗病毒治疗慢性乙型肝炎(chb)不同应答者肝组织中stat-1分子表达的差异.方法 选取对ifnα抗病毒治疗完全应答chb患者5例(a组)、无应答者8例(b组),提取所选患者在抗病毒治疗前和治疗结束时的肝穿组织.对所取肝组织进行病理学检测、stat-1mrna经rt-pcr半定量检测,并用免疫印迹法检测肝组织中的stat-1蛋白水平.结果 a组肝组织stat-1 mrna吸光度值(a)为(1.18±0.06),b组为(0.21±0.04),两者比较差异有统计学意义(t=35.27,p<0.01).a组患者肝组织中stat-1蛋白水平明显高于b组.结论 chb患者发生ifn抵抗可能与stat-1表达显著下调有关.; abstract：; objective to investigate the expression of signal transduction molecule stat-1 in patients with chronic hepatitis b (chb) who received interferon-alpha (ifnα) treatment with different responses. methods a total of 13 chb patients received ifnα treatment, among who 5 demonstrated complete viral response ( group a) and 8 showed no response ( group b). all patients underwent liver biopsy before and after the therapy. stat-1 mrna of liver tissue was semi-quantified by rt-pcr and levels of stat-1 protein were detected by western-blot. results absorbance values (a) of stat-1 mrna in group a and b were (1.18±0.06) and (0.21±0.04) respectively (t =35.27, p<0.01). the levels of stat-1 pr...

• 单位
  武汉大学人民医院; 病毒学国家重点实验室