摘要

目的建立测定脑安颗粒中阿魏酸含量的方法。方法采用反相高效液相色谱法(RP-HPLC法),色谱柱为PhenomenexC18柱(250mm×4.6mm,4μm),以甲醇-水-冰醋酸(30∶70∶1)为流动相,流速为0.85mL/min,柱温为15℃,检测波长为323nm。结果阿魏酸进样量在0.117~2.340μg范围内与峰面积线性关系良好,r=0.9999,回收率为98.92%,RSD为1.27%(n=6)。结论RP-HPLC法操作简便,测定结果准确,可用于脑安颗粒中阿魏酸含量的测定。

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