Vybrant DyeCycle Violet Stain Discriminates Two Different Subsets of CD34+Cells

作者：Nunez Espinosa Cristian; Garcia Godoy Maria D; Ferreira Ines; Rios Kristjansson Juan G; Rizo Roca David; Rico Laura G; Rubi Sans Gerard; Palacio Carlos; Torrella Joan R; Pages Teresa; Ward Mike D; Viscor Gines; Petriz Jordi^*

来源：Current Stem Cell Research and Therapy, 2016, 11(1): 66-71.

DOI：10.2174/1574888X10666150528152547

• CD34
• flow cytometry
• P-glycoprotein
• Stem cells
• Vybrant DyeCycle Violet

Introduction: Studies are needed to understand the role of CD34 expressing cells with regard to efficient engraftment, especially in the adjuvant treatment of cancer. Materials and Methods: In this study we have used a modified method in our laboratory for routinely counting CD34+ cells. Unlysed whole blood samples were stained with the DNA-selective and cell membrane-permeant Vibrant DyeCycle Violet stain. Results: CD34+ cells exhibit a consistent and differential Vybrant Dye Cycle Violet staining pattern. Based on their different DCV intensity, we classified these subpopulations as CD34+/DCV high and CD34+/DCV low cells. In general, DCV high cells are about 12-times brighter than DCV low cells. Conclusion: DCV staining may be used to discriminate subsets of CD34+ cells similarly to other methods which have previously defined different functional properties that can be related to the characterization, resolution, and purification of primitive hematopoietic stem cells in combination with specific useful markers for multicolor flow cytometric measurements.