Avaliaˋˋo de um modelo experimental de enterocolite necrosante neonatal em ratos

作者：Meyer; Karine Furtado; Martins; Jose Luiz; Freitas Filho; Luiz Gonzaga de; Oliva; Maria Luiza Vilela; Patricio; Francy Reis da Silva; Macedo; Mauricio; Wang; Lina

来源：Acta Cirurgica Brasileira, 2006.

DOI：10.1590/S0102-86502006000200011

• enterocolitis
• necrotizing
• models
• animal
• rats.

objective: to evaluate an experimental model of necrotizing enterocolitis in rats proposed by okur e col. in 1995. methods: on their first day of life, 28 epm-wistar rats weighing between 4 and 6 grams were submitted to hypoxia (h) by placing them in a co2 gas chamber for rodents%26apos; sacrifice, where they received a 100% co2 air flow for 5 minutes. after the hypoxia the animals were reanimated (r) with a 100% o2 air flow, also for 5 minutes. the animals were allocated in two groups: g1: control (n=12): rats not submitted to h-r; g2: (n=16): rats submitted to h-r. segments of the small intestine and colon were prepared for histological analysis. the remaining intestine was used to measure tissular malondialdehyde. results: mean malondialdehyde dosages were 1.05 (0.44-2.03) and 2.60 (0.59- 6.4) nmol mda/mg protein for g1 and g2, respectively. g2%26apos;s mean value was significantly higher than in the control group (p%26lt;0.002). significant statistical difference between the studied groups was found in relation the level of injury, with g1 presenting significantly lower levels than g2. conclusions: the model showed that neonatal hypoxia may cause intestinal wall injury in rats. despite the discreet histological injuries found, the method is suitable for evaluation of tissular free radicals.