The development of an iridium(Ⅲ) complex functionalized G-quadruplex probe for the stability of G-quadruplex and lifetime image in cytoplasm

作者：Shaokang Jia; Wenjin Wang; Shanshan Qin; Shengjie Xie; Lisi Zhan; Qi Wei; Ziang Lu; Xiaolu Zhou; Cong Chen; Kun Chen; Shen Yan; Caiping Tan; Zongwan Mao^*; Xiang Zhou^*

来源：Chinese Chemical Letters, 2023, 34(02): 429-434.

• G-quadruplex
• Pyridostatin derivatives
• Iridium(Ⅲ) complex
• Phosphorescent probe
• Phosphorescence lifetime
• Cell imaging

G-quadruplex(G4) is widely known as a non-classical secondary structure of nucleic acid. With the indepth study of G4, it is an urgent need for a phosphorescent probe with a high G4 binding ability to evaluate the level of G4 in the cytoplasm. Thus, this study designed and synthesized Ir-PDP where an Ir(Ⅲ)complex was used as a phosphorescent emitter. Meanwhile, two installed PDPs(pyridostatin derivatives)were used to improve the combination ability with G4 and reduced the cytotoxicity of the Ir(Ⅲ) complex.Compared with other nucleic acid secondary structures, Ir-PDP produced a higher phosphorescence lifetime after interacting with G4. Ir-PDP was distributed in the cytoplasm of living cells, and two-photon phosphorescence lifetime imaging can detect the binding events of the probe in the cytoplasm. The addition of G4 binder PDS significantly regulated cytoplasmic phosphorescence lifetime. The project explored a new sensing pathway to observe the binding manners of probes in the cytoplasm through the phosphorescence lifetime of probes.

• 单位
  中山大学