Utilization of low-cost substrates for the production of nystose by Bacillus subtilis natto cct 7712

作者:dos Santos Dieyssi Alves; Baldo Cristiani; Borsato Dionisio; Pedrine Colabone Celligoi Maria Antonia
来源:Acta Scientiarum - Technology, 2016, 38(4): 391-397.
DOI:10.4025/actascitechnol.v38i4.28704

摘要

Current analysis describes the capacity of Bacillus subtilis natto CCT 7712 to produce high amounts of nystose by low-cost substrates available in Brazil, such as commercial sucrose, sugarcane molasses and sugarcane juice. Optimization resulted in a maximum production of 179.77 g L-1 of nystose, averaging 7.49 g L-1 hour(-1) of productivity and a 71.73% yield in a medium with 400 g L(-1)of commercial sucrose and 0.8 g L-1 of MnSO4. Fermentations with sugarcane molasses and sugarcane juice also resulted in a satisfactory production reaching 97.93 and 42.58 g L-1 nystose, respectively. High nystose production in a medium with sugarcane derivatives suggests submerged fermentation with Bacillus subtillis natto CT 7712 as a promising strategy to produce nystose at industrial level.

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