The Effects of IFN-污 on Expression of IL-18 in Uterus and Ovaries and IL-18 Levels in Peripheral Blood of Abortion Rats

作者：Si Li Fang; Chen Shu Lin; Cheng Xiang Chao; Qing Su Zhu; Si Xian Li; Zhang Xiao Ying; Wang Jin Yi; Xu Yong Ping; Fan Guang Li

来源：Journal of Animal and Veterinary Advances, 2012.

DOI：10.3923/javaa.2010.2194.2199

To explore the immunological regulatory mechanisms of IFN-污 on IL-18 and its effect on pregnancy, the dynamic expression of mRNA and cellular localization of protein for IL-18 in the early pregnant rats after the injection of different doses of IFN-污 were observed by RT-PCR and immunohistochemistry. ELISA assay was also applied to investigate the effect of exogenous IFN-污 on the level of IL-18 in the peripheral blood. The results indicated that IL-18 mRNA expression level was the highest in uterus and ovaries of normal pregnancy group and the lowest in abortion model group; compared with the abortion model group, the IL-18 mRNA expression level was up-regulated both in 2.4 and 2.8 IU IFN-污 dose group. Howere, no significant difference can be seen between 2 IU IFN-污 dose group and the normal pregnancy group (p%26gt;0.05). Furthermore, IL-18 protein was strongly expressed in the normal pregnant group and 2IU IFN-污 group. ELISA results showed that the level of IL-18 in peripheral blood of abortion model was the lowest and it was extremely significantly different from normal pregnancy group and 2 IU IFN-污 group (p%26lt;0.01).