Terapia fotodinamica com ftalocianina de zinco t車pica: avaliaˋˋo da intensidade de fluoresc那ncia, absorˋˋo cutanea, alteraˋˋes histol車gicas e imuno-histoqu赤micas na pele do modelo animal

作者：Tomazini; Marilia Vannuchi; Souza; Cacilda da Silva; Garcia; Sergio Britto; Tedesco; Antnio Claudio

来源：Anais Brasileiros de Dermatologia, 2007.

DOI：10.1590/S0365-05962007000600006

• apoptosis
• immunohistochemistry
• lasers
• models
• animal
• photochemotherapy
• skin absorption
• spectrometry
• fluorescence.

background - phthalocyanines are promising photosensitizers used in photodynamic therapy (pdt). objectives - to evaluate the following parameters: intervals, vehicles and enhancer using topical zinc-phthalocyanine (zn-pc) formulation. to examine macro and micromorphological changes and fas expression induced by topical zn-pc-pdt on murine skin. material and methods - using fluorescence spectrometry, different intervals of topical occlusion employing zn-pc gel or emulsion, with or without monolein 5% were studied. after an 8-hour occlusion of different formulations, the mice were exposed to 670 nm laser, at a 50 j.cm-2 dose. results - fluorescence was slightly higher after 8 hours, and also with emulsion formulation at one-, two- and four-hour occlusion periods. the intensity of edema and erosion were correlated to epidermal necrosis and to fas immunoexpression in skin histological specimens. conclusions - the results show the effects of photodynamic action promoted by the interaction between zn-pc formulation and a 670-nm light source. macro and micromorphological alterations were correlated and more substantial with monolein and zn-pc emulsion, suggesting more marked effects with this formulation. the fas immunoexpression and histological changes suggested that apoptosis plays a role in the mechanism of cell death caused by pdt based in zn-pc.