科研之友
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摘要
Objective:To explore the pharmacological anti-inflammatory mechanism of Chinese formula Qingwen Baidu Decoction(清瘟败毒饮,QBD) from the view of holistic biology.Methods:The rats were randomly divided into a normal conrol group,a lipopolysaccharide(LPS) group,the low- and high-dose QBD groups,and a dexamethasone(DXM) group.NR8383 cells were treated with culture fluid containing 6%serum from rats of each group respectively.Inflammatory mediators were detected by reverse transcription polymerase chain reaction(RT-PCR),Western blotting hybridization,enzyme linked immunosorbent assay(ELISA),polymerase chain reaction(PCR) gene array and antibody array.Results:It is showed that the levels of interleukin(IL)-1 α,IL-4 and IL-12 were enhanced in the low-dose QBD group;levels of IL-1 α,IL-12 and IL-18 were augmented in the high-dose QBD group,compared with the LPS group after ELISA detection.Western blot showed that IL-1β and tumor necrosis factor(TNF)-α expression of the control group were lower than other groups.IL-1 βlevel of the low-dose and high-dose QBD groups detected by RT-PCR was higher in early stage but lower after24 h than that of the control group(P<0.01).Expression of 84 main inflammatory cytokines and receptors was detected by rat inflammatory cytokines and receptors PCR array.Up-regulation genes were 22 in both the LPS group and the low-dose QBD group,among which 16 up-regulating genes were the same.In these 16 genes,the up-regulating amplitude of 9 genes in the low-dose QBD group was less than that in the LPS group,4 were similar to and 3 were more.Twenty-nine main cytokines were inspected by rat cytokine antibody array.Intergroup gray value differences were found in 7 expressed cytokines.The levels of these 7 cytokines in the lowdose QBD group were all lower than those in the the LPS group.Conclusions:QBD has anti-inflammatory effect on sepsis by changing the level of inflammatory mediators.
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