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摘要
Objective To study the indole alkaloids from the roots of Rauvolfia yunnanensis, and its antibacterial activities, so as to clarify the antibacterial active ingredients of the plant. Methods The constituents were isolated and purified by silica gel column chromatography (CC), RP-C18, Sephadex LH-20 and semi-preparative chromatography, their structures were identified by the combination of physicochemical properties, spectral data and literature comparison. Then, the antibacterial activities of these compounds were detected by the method of micro broth dilution. Results Totally twenty indole alkaloids were obtained and identified as vinorine (1), perakine (2), tetraphyllicine (3), raugalline (4), sitsirikine (5), geissoschizol (6), corynantheol (7), dihydrocorynantheol (8), antirhine (9), isoantirhine (10), pelirine (11), 10-hydroxy-16-epi-affinine (12), yohimbine (13), normacusine B (14), lochnerine (15), akuammidine (16), deacetylakuammiline (17), reserpine (18), harman (19) and melinonine F (20). Moreover, compound 20 showed significant antibacterial activity against Canidia albicans with MIC value of 3.12 μg/mL. Compounds 1 and 11 showed certain antimicrobial activity against Escherichia coli, and compounds 11, 13, and 20 showed certain antimicrobial activity against Bacillus subtilis, the MIC values were 6.25—12.50 μg/mL respectively. And their activities were comparable to antibiotics berberine. Conclusion Compound 20 was firstly reported from the genera Rauvolfia, and compounds 6, 8—13, 16—18 were firstly reported from this plant. Some compounds showed potential antimicrobial activities. ? 2023 Editorial Office of Chinese Traditional and Herbal Drugs.
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