Highly Sensitive and Selective Detection of Food Toxin Using Three Functional DNA Hairpins

作者：Sui, Zhe; Wu, Wei; Komiyama, Makoto^*; Liang, Xingguo^*

来源：Chemistry Letters, 2018, 47(8): 1026-1028.

DOI：10.1246/cl.180357

• Aptamer
• DNA hairpin
• G-quadruplex

By combining three kinds of DNA hairpins (one involving both aptamer sequence and trigger sequence, and the other two to amplify the signal by hybridization chain reaction), a food toxin ochratoxin A (OTA) was selectively detected with a remarkably high sensitivity. The signal was visualized with the use of the peroxidase-like function of G-quadruplexes that were formed from the terminal sequences of two adjacent fragments in the DNA nanowire. Even 0.08 ppb of OTA was successfully detected.

• 单位
  中国海洋大学; 青岛大学