Fertiliza o e desenvolvimento embrion芍rio: morfometria e an芍lise estereomicrosc車pica dos ovos dos h赤bridos de surubins (pintado, Pseudoplatystoma corruscans x cachara, Pseudoplatystoma fasciatum) = Fertilization and embrionary development: morphometry and stereomicroscopic analysis of hybrids＊ eggs from surubins (pintado, Pseudoplatystoma corruscans and cachara, Pseudoplatystoma fasciatum)

作者：Francine Faustino; Laura Satiko Okada Nakaghi; Camila Marques; Lilian Cristina Makino

来源：Acta Scientiarum - Biological Sciences, 2007.

• embriogenese
• hibrido
• surubins
• morfometria
• Embriogenesis
• hybrid
• surubins
• morphometry

O objetivo deste estudo foi analisar a morfometria dos ovos dos h赤bridos sob estereomicrosc車pio. Foi realizada a reprodu o induzida em exemplares de f那meas de pintado (P. corruscans) e de machos de cachara (P. fasciatum) do Cepta - Ibama, Pirassununga, Estado de S o Paulo, Brasil. As amostras foram coletadas no momento da extrus o, durante a fertiliza o, nos tempos 10 e 30 segundos, 1, 2, 5, 8, 10, 15, 20, 30 e 45 minutos, 1 hora, a cada 15 minutos at谷 completar 2 horas e depois a cada hora at谷 a eclos o da larva. Ov車citos e ovos foram observados quanto 角 morfologia externa, fotografados e 30 amostras de cada momento foram medidas em estereomicrosc車pio. Os ov車citos, no momento da extrus o, eos ovos apresentaram formato esf谷rico e colora o amarelada. As caracter赤sticas observadas foram: amplo espa o perivitel赤nico, somitos, presen a da ves赤cula. As principais fases do desenvolvimento embrion芍rio como c谷lula-ovo (blastodisco), 2, 4, 8, 16, 32 e 64 blast meros, m車rula, bl芍stula, g芍strula e eclos o da larva tamb谷m foram verificadas. This study aimed to analyze the morphometry of hybrids＊ eggs under stereomicroscopy. The induced reproduction was carried out in specimens of pintado＊s females (P. corruscans) and cachara＊smales (P. fasciatum) from Cepta - Ibama, Pirassununga, state of S o Paulo, Brazil. Samples were collected in extrusion moment, during the fertilization, in times of 10 and 30 seconds, 1, 2, 5, 8, 10, 15, 20, 30 and 45 minutes, 1 hour, at every 15 minutes until completing 2hours, and afterwards, at every hour until the larval hatching. Oocytes and eggs＊ external morphology were observed, photographed and 30 samples from each moment were measured in stereomicroscopy. Oocytes, in the time of extrusion, and the eggs presented aspherical form and yellowish color. Characteristics such as wide perivitelinic space, somites, presence of optical vesicle and the main phases of embryonic development as egg-cell (blastodisc), 2, 4, 8, 16, 32 and 64 blastomeres, morula, blastula, gastrula, epibolymovements, formation and larvae hatching were verified.