科研之友
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摘要
introduction: human metapneumovirus (hmpv) is responsible for respiratory infections in children and immunocompromised adults and elders. it is commonly diagnosed by immunofluorescence or molecular biology. objective: to detect hmpv in clinical samples by polymerase chain reaction (pcr) and direct imunofluorescence (dif) methods. results: two percent of 202 samples were positive for dif and 4% of them for reverse transcriptase pcr (rt-pcr), respectively. considering rt-pcr as gold standard, dif sensitivity and specificity were 50% and 100%, respectively. conclusion: not only does the study show that rt-pcr is the best method for hmpv detection in clinical respiratory samples but it also substantiates the importance of test standardization in laboratory routine.
