科研之友
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摘要
The objective of this study was to determine the synergistic effects of beta-Mercaptoethanol (汕-ME) and MEM vitamins (MEMv) as antioxidants supplemented into porcine maturation medium on maturation and Reactive Oxygen Species (ROS) of oocytes. In addition, the effects of 汕-ME and MEMv on subsequent embryonic development, ROS generation by embryos and apoptosis of blastocysts following In Vitro Fertilization (IVF), Chemical Activation (CA) and Electrical Activation (EA) were investigated. Cumulus-Oocyte-Complexes (COCs) were cultured in North Carolina State University (NCSU)-23 medium supplemented with 0.05xMEMv, 25 米M 汕-ME and 25 米M 汕-ME and 0.05xMEMv, respectively. COCs cultured without 汕-ME and MEMv were regarded as control. Maturation rate and ROS levels of oocytes at 44 h of culture were assessed. Oocytes were fertilized in vitro or activated parthenogenetically and embryonic development and the ROS levels on 2 days of culture and the apoptosis levels of blastocyts were evaluated. Maturation rate of the 汕-ME and MEMv group was higher than that of the control group (86.0㊣3.6 vs. 73.3㊣1.1%; p%26lt;0.05) although, ROS levels were not significantly different among the groups. Cleavage rate following IVF was increased in the 汕-ME and MEMv group compared to those of other groups and ROS levels of the 汕-ME and MEMv group were lower than those of the control group (6.9㊣0.2 vs. 8.0㊣0.2; p%26lt;0.05). Blastocyst rate was increased in the 汕-ME and MEMv group compared to those of the control and MEMv groups (p%26lt;0.05). Conversely, the level of apoptosis of the 汕-ME and MEMv group was lower than others (4.6㊣1.0 vs. 11.4㊣1.9%, 12.2㊣1.8 and 15.6㊣3.0%, respectively; p%26lt;0.05). Cleavage rate of the 汕-ME and MEMv group following CA was significantly higher compared with control while the ROS level was lower than the 汕-ME group (19.0㊣1.9 vs. 25.0㊣1.7; p%26lt;0.05). The blastocyst formation rate of the 汕-ME and MEMv group was significantly increased than control while the level of apoptosis was lower than those of other groups (5.8㊣1.2 vs. 17.2㊣4.6%, 17.0㊣5.2 and 22.6㊣3.2%, respectively; p%26lt;0.05). Embryonic developments following EA were not significantly different among the groups although, the ROS level of the 汕-ME and MEMv group was lower (p%26lt;0.05). In conclusion, addition of 汕-ME and MEM vitamins during porcine oocyte maturation was effective on subsequent embryonic development in IVF and CA by alleviating the influences of ROS generation and apoptosis.
