科研之友
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摘要
The extracellular matrix is a key element in the tracheal morphology and physiology, both in normal and pathological states, yet little is known regarding its composition and organization. Herein we carried out a detailed study of the morphological organization and volumetric density of elastic system fibers in the trachea of Wistar rats. Six Wistar rats at the age of 45 days were used. The trachea was excised following anesthesia with Thiopental and perfusion through the left ventricle with buffered saline followed by formalin solution. Samples were fixed in formaline, embedded in paraffin, and histologically processed. The elastic system fibers were evaluated at light microscopy by using Weigert%26apos;s resorcin-fuchsin technique after oxidation with oxone. Morphometric studies were performed by the point-counting method. Quantities were expressed (%, mean) as volumetric densities and were determined on 25 random fields for each animal. Elastic system fibers were identified beneath the tracheal mucosa showing mainly a longitudinal profile. There were also some bundles of oblique fibers in this region, forming an irregular network of elastic tissue. Close to the inner surface of the cartilaginous ring, a well defined circular layer is present. The volumetric density of the elastic system fibers in the trachea of Wistar rats is 2.46 ㊣ 0.99%. The data in the present study provides original information regarding the elastic system fibers of the rat trachea, which might be used as a model for studying human major airway morphology. The results reported herein provide the basis for continuous investigations on tracheal extracellular matrix by stereology. La matriz extracelular es un elemento clave en la morfolog赤a y fisiolog赤a de la tr芍quea, tanto en estados normales como patol車gicos. Sin embargo, poco se sabe acerca de su composici車n y organizaci車n. Se llev車 a cabo un estudio detallado de la organizaci車n morfol車gica y la densidad volum谷trica de fibras del sistema el芍stico en la tr芍quea de ratas Wistar. Se utilizaron seis ratas Wistar edad de 45 d赤as de vida. La tr芍quea fue extra赤da despu谷s de anestesia con tiopental y la perfusi車n a trav谷s del ventr赤culo izquierdo con soluci車n salina tamponada seguido de soluci車n de formalina. Las muestras fueron fijadas en formalina e incluidas en parafina y se procesaron histol車gicamente. Las fibras del sistema el芍stico fueron evaluadas en microscop赤a de luz utilizando la t谷cnica de Weigert, resorcina-fucsina despu谷s de la oxidaci車n con Oxone. Los estudios morfom谷tricos fueron realizados por el m谷todo de conteo de puntos. Las c
