海南长春花黄化病植原体的16S rDNA序列分析研究

作者：车海彦; 罗大全; 符瑞益; 叶莎冰; 吴云锋

来源：植物病理学报, 2009, 39(02): 212-216.

DOI：10.3321/j.issn:0412-0914.2009.02.015

• periwinkle yellows disease
• Phytoplasma
• 16S rDNA
• sequence analysis

Periwinkle(Catharanthus roseus) yellows is a common disease in Hainan. Periwinkle′s leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers (Rl6mF2/Rl6mR1). A PCR product (about 1.4 kb) was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group (16S r Ⅴ) and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma (PY-Hn). This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle.

• 单位
  西北农林科技大学; 中国热带农业科学院环境与植物保护研究所