Deletion of NEDD4‐2 Abolishes the Inhibitory Effect of High Sodium (HS) Intake on the Basolateral Kir4.1/Kir5.1 of the DCT and Thiazide‐Sensitive Na‐Cl Cotransport (NCC)

作者：Zhang Dandan; Duan XinPeng; Wu Peng; Xiao Yu; Gao ZhongXiuzi; Wang WenHui; Lin DaoHong

来源：The FASEB Journal, 2020, 34(S1): 1-1.

DOI：10.1096/fasebj.2020.34.s1.02532

Background We have previously demonstrated that the deletion of NEDD4‐2 increased the basolateral Kir4.1/Kir5.1 channel activity in the distal convoluted tubule (DCT), suggesting the role of NEDD4‐2 in the regulation of basolateral Kir4.1/Kir5.1. Also, it has been demonstrated that dietary sodium intake regulated the basolateral Kir4.1/Kir5.1 channel activity in the DCT such that low sodium (LS) intake stimulated whereas HS intake inhibited the Kir4.1/Kir5.1 channels in the DCT. The HS‐induced inhibition of basolateral Kir4.1/Kir5.1 channels in the DCT was essential for the HS‐induced inhibition of NCC expression/activity because HS failed to inhibit NCC in the kidney‐specific Kir4.1 knockout mice. The aims of the present study are: 1) To explore whether the effect of HS on the basolateral Kir4.1/Kir5.1 in the DCT is compromised in the absence of Nedd4‐2; 2) To examine whether the deletion of Nedd4‐2 abolishes the effect of HS on NCC activity. Methods We have used the patch‐clamp methods to measure the basol...

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  New york medical college; new york medical college