The Profiling of Circulating Tumor DNA in Pediatric Mature B-Cell Non-Hodgkin Lymphoma (B-NHL): A Multicenter and Prospective Clinical Study

作者：Wang, Juan; Guo, Haixia; Yun, Cai; Chen, Huiqin; Yang, Liangchun; Dou, Huihong; Tian, Xin; Xiong, Hao; You, M. James; Hong, Xiaoyu; Liang, Chuqiao; Zhen, Zijun; Zhu, Jia; Lu, Suying; Sun, Feifei; Huang, Junting; Zhang, Yizhuo

来源：Blood, 2021, 138(S1): 2400-2400.

DOI：10.1182/blood-2021-151686

Background(#br)Next-generation sequencing (NGS) based on liquid biopsy has been an emerging technology to identify tumor-specific genetic aberrations in adult lymphoma. However, there were few studies on the genomic profiling of plasma circulating tumor-derived DNA (ctDNA) in pediatric mature B-NHL.(#br)Methods(#br)Paraffin-embedded tissue (FFPE) and plasma samples from the newly diagnosed patients were collected and sequenced by 475 genes panel before, during and post of treatment. Clinical stage system, risk stratification and treatment of pediatric mature B-NHL followed a the modified BFM-95 protocol.(#br)Results(#br)A total of 53 pediatric mature B-NHLs were enrolled，including 35 Burkitt lymphoma, 18 diffused large B cell lymphoma/high-grade B cell lymphoma (DLBCL/HGBCL). We collected 38 tissue and 124 plasma samples for somatic mutation testing. The number of somatic mutations and the TOP 5 genes detected in the 38 tissues and 31 baseline plasma samples, were 416 vs 496, and MYC(71%), DDX3X(45%)，ID3 (42%...

• 单位
  华南肿瘤学国家重点实验室; 中山大学; 南方医科大学; 中南大学; 华中科技大学