一种用高拷贝质粒载体制备BAC载体基本功能基因的新方法(英文)

作者：崔红玉; 王亚萍; **举; 薛永志; 石星明; 兰德松; 王云峰; 童光志

来源：Agricultural Science & Technology, 2008, (02): 70-75.

DOI：10.16175/j.cnki.1009-4229.2008.02.003

• Bacterial artificial chromosome (BAC)
• High-copy plasmid
• BAC vector
• Single-copy plasmid Bacterial artificial chromosome (BAC)
• Single-copy plasmid

[Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119-Bluelox BAC.[Method] With selecting a proper single restriction site,sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plas...

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  兽医生物技术国家重点实验室; 动物科技学院; 河北北方学院; 中国农业科学院哈尔滨兽医研究所