Genetic Analysis of Triple‐Negative Breast Cancer Cell Lines and the Therapeutic Role of Akt/Nrf2 Crosstalk in Resistance to EGFR Inhibitors

作者：Parham Jabbarzadeh Kaboli; Yao Chen; Shixin Xiang; Zhangang Xiao

来源：The FASEB Journal, 2020, 34(S1): 1-1.

DOI：10.1096/fasebj.2020.34.s1.03864

Introduction(#br)Triple‐Negative Breast Cancer (TNBC) is the most incurable subtype of breast cancer. Tang and Epidermal growth factor receptor (EGFR), which is a well‐known tyrosine kinase receptor, is expressed in 50% of TNBC cells. Chemotherapy with tyrosine kinase inhibitors (TKIs) has been recognized as the most important therapeutic method against TNBCs. TNBC patients showed resistance to EGFR targeted therapy. Recently, we studied the antitumor effects of berberine in MDA‐MB231 TNBC and MCF‐7 cells (1) . We would like to report our unexpected results and the predicted association between different pathways and resistance to chemotherapy.(#br)Material and methods(#br)To explain the mechanism of resistance to lapatinib and other EGFR inhibitors, we analyzed genetic profiles of ten TNBC cell lines. We selected 35 genes from different pathways which are involved in resistance including Akt signaling, Nrf2 anti‐oxidant signaling, and TGF‐β/SMADs pathway together with PD‐L1, CD28, CD80, and CD86.(#br)Results...