The expression and clinical significance of neuron specific enolase and S100B protein in patients of severe heatstroke-induced brain injury [重 症 中 暑 脑 损 伤 患 者 神 经 元 特 异 性 烯 醇 化 酶 及 脑 活 性 肽100B蛋白的表达及意义]

作者：Bing L.; Ye-Ran J.; Wei G.; Hui-Ping L.; Wei-Hua T.; Hong-Xuan Z.^*

来源：Medical Journal of Chinese People's Liberation Army, 2020, 45(12): 1282-1287.

DOI：10.11855/j.issn.0577-7402.2020.12.12

• Brain injury
• Glasgow coma scale
• Neuron specific enolase
• S100B
• Severe heatstroke

[Abstract] Objective To study the expression of neuron specific enolase (NSE) and S100B protein in patients with severe heatstroke-induced brain injury and its clinical significance, changes in the degree of damage. Methods Ninety-six patients with heatstroke-induced brain injury in Shenzhen People's Hospital from May 2016 to July 2019 were enrolled and classified into the mild coma group (13-14, n=32), the moderate coma group (9-12, n=32) and the severe coma group (3-8, n=32) according to the GCS, randomly, with another 96 healthy adults serving as controls. The serum and cerebrospinal fluid levels of NSE, S100B in patients with heatstroke-induced brain injury at 0 h, 1 d, 3 d, 7 d after injury were measured with ELISA and Western blotting. The correlation between the dynamic changes of NSE, S100B and the degree of injury and poor prognosis of GCS score were analyzed. Results The levels of NSE, S100B in serum and cerebrospinal fluid of control group was obviously lower than those in heatstroke groups, and the differences were statistically significant (P<0.05). The levels of NSE, S100B in serum were obviously lower than those in cerebrospinal fluid sample in the same damages degree groups (P<0.05). In the different damage degree groups, serum and cerebrospinal fluid NSE, S100B levels were significantly higher in moderate group than those in mild group (P<0.05), while these indexes in severe group were significantly higher than those in moderate group (P<0.05). By analysing the dynamic changes of S100B levels in serum and cerebrospinal fluid in the three different damages degree groups, the mild group reached the peak at 1 d, the moderate group and the severe group reached the peak at 3 d, while the three groups of NSE reached the peak at 3 d. There were positive correlations between NSE and S100B levels; while negative correlations between NSE levels and GCS, S100B levels and GCS, respectively (P<0.05). Conclusion The significantly increased expression of NSE and S100B is closely related to the severity of heatstroke-induced brain injury, can reflect the degree of brain tissue injury, and may serve as objective indicators in the early prediction of heatstroke-induced brain injury.

• 单位
  暨南大学