Extraction and anticoagulant activity of sulfated polysaccharides from Caulerpa cupressoides var. lycopodium (Vahl) C. Agardh (Chlorophyceae) = Extra o e atividade anticoagulante dos polissacar赤deos sulfatados da clorof赤cea Caulerpa cupressoides var. lycopodium (Vahl) C. Agardh = Extra o e atividade anticoagulante dos polissacar赤deos sulfatados da clorof赤cea Caulerpa cupressoides var. lycopodium (Vahl) C. Agardh.

作者：Jose Arievilo Gurgel Rodrigues; Edfranck de Sousa Oliveira Vanderlei; Ana Luiza Gomes Quindere; Bruno Pedrosa Fontes

来源：Acta Scientiarum - Biological Sciences, 2011.

• chlorophyta
• sequential extractions
• physical-chemical characterization
• coagulation test
• cloroficea
• extra es sequenciais
• caracteriza o fisico-quimica
• teste de coagula o

The reportedly low standard quality of heparin (HEP) for use in cardiac surgeries has led to concern in the Brazilian and international markets. Sulfated polysaccharides (SPs) from seaweeds have been regarded as promising substitutes for HEP. The aim of this study was to sequentially extract total SPs (TSPs) from Caulerpa cupressoides (Chlorophyceae) with papain in 100 mM sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed byfractionation by ion-exchange chromatography (DEAE-cellulose), and then evaluate the anticoagulant potential of SP fractions by activated partial thromboplastin time (APTT) using normal human plasma and compare it to standard HEP (193 IU mg-1). The obtained fractions were chemically characterized by chemical composition and agarose gel electrophoresis. The yield was 4.61%, and three fractions of SP (F I, F II and F III) eluted with 0.50, 0.75 and 1.00 M of NaCl,respectively, were observed on chromatography profiles; however, differences in charge densities patterns and degree of resolution among them were revealed by electrophoresis. SPs were capable of modifying APTT only in fractions eluted with 0.75 M of NaCl, whose activities were 23.37 and 25.76 IU mg-1, respectively, and the charge density was prerequisite to activity. Therefore, C. cupressoides is a source of SPs possessing low anticoagulant potential compared to HEP. O baixo padr o de qualidade outrora declarado da heparina (HEP) para o uso em cirurgias card赤acas tem levado preocupa o nos mercados nacional e internacional. Os polissacar赤deos sulfatados (PSs) de algasmarinhas t那m sido considerados como promissores substitutos para HEP. Objetivou-se a extrair sequencialmente PSs totais (PSTs) da clorof赤cea Caulerpa cupressoides com papa赤na em tamp o acetato de s車dio 100 mM (pH 5,0) contendo ciste赤na 5 mM e EDTA 5 mM, fracionar por cromatografia de troca i nica (DEAE-celulose) e avaliar o potencial anticoagulante das fra es de PS por meio do tempo de tromboplastina parcial ativada (TTPA), utilizando plasma humano normal e comparando-se 角 HEP padr o (193 IU mg-1). As fra es obtidas foram caracterizadas quimicamente em composi o qu赤mica e por eletroforese em gel de agarose. O rendimento de PSTs foi 4,61% e os perfis cromatogr芍ficos, em DEAE-celulose, indicaram a separa o de tr那s fra es de PS (F I; F II e F III) elu赤das nas concentra es 0,50; 0,75 e 1,00 M de NaCl, respectivamente, revelando, por eletroforese, diferen as em termos de densidade de cargas e graude resolu o. Os PSs foram capazes de modificar o TTPA somente nas fra es elu赤das com 0