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摘要
Objetivou-se avaliar diferentes concentra es de nitrato de c芍lcio e nitrato de am nio no crescimento in vitro de orqu赤dea. Plantulas de Cattleya loddigesii oriundas de sementes germinadas in vitro, com 1,0 cm de comprimento, foram inoculadas em frascos contendo 60 mL de meio de cultura WPM modificado em suas concentra es de nitrato dec芍lcio (0, 278, 556, 834 e 1112 mg L-1) e nitrato de am nio (0, 200, 400, 600 e 800 mg L-1). O meio foi acrescido de 20 g L-1 de sacarose, 150 g L-1 de polpa de banana ＆nanica＊ madura e 2 g L-1 de carv o ativado, pH ajustado para 5,7 ㊣ 0,1 e solidificado com 6 g L-1 de 芍gar, antes da autoclavagem a 121oC e 1,5 atm, por 20 min. Ap車s a inocula o, os frascos foram transferidos para sala de crescimento a 25 ㊣ 2oC, fotoper赤odo de 16h e 35 米mol m-2 s-1 de intensidade luminosa. Decorridos 90 dias, observaram-se melhores resultados para n迆mero de folhas e de brotos com 400 e 450 mg L-1 de nitrato de am nio, respectivamente, enquanto que o maior n迆mero de ra赤zes foi obtido com 600 mg L-1 de nitrato de am nio e 278 mg L-1 de nitrato de c芍lcio. Recomenda-se a utiliza o do meio WPM em sua composi o original, sem nitrato de c芍lcio na micropropaga o desta esp谷cie. This work aimed to evaluate the effect of different concentrationsof calcium and ammonium nitrate on the in vitro development of orchids. Cattleya loddigesii orchid plantlets, 1.0 cm in size produced by self pollinization and also by in vitro germinatedseeds, were inoculated in flasks containing 60 mL of WPM culture medium, modified with different concentrations of calcium nitrate (0, 278, 556, 834 and 1112 mg L-1) and ammonium nitrate (0, 200, 400, 600 and 800 mg L-1). The culture medium was supplemented with 20 gL-1 of sucrose, 150 g L-1 of ＆nanica＊ banana pulp, activated charcoal 2 g L-1, solidified with agar 6 g L-1, and pH adjusted to 5.7 ㊣ 0.1 before being autoclaved at 121oC, 1.5 atm pressure during 20 minutes. After inoculations, the flasks were transferred to a growth room with controlled temperature around 25 ㊣ 2oC, 16h photoperiod regime, with a light intensity of 35 米mol m-2 s-1. After 90 days, it was observed that the best results in terms of number of leaves was achieved with 400 mg L-1 of ammonium nitrate, and the highest number of sprouts was obtained with 450 mg L-1 of ammonium nitrate, but the majornumber of roots was verified in the treatment with 600 mg L-1 of ammonium nitrate and 278 mg L-1 of calcium nitrate. In resume, it is recommended to use the WPM medium in its original composition, without calcium nitrate to micropropagate C. loddigesii orchidp
