Prenatal Stress Caused by Movement Restriction Induces Changes in the Appendicular Osseous Development of CF-1 Mice Progeny El Estr谷s Prenatal Causado por Restricci車n de Movimiento Induce Cambios en el Desarrollo 車seo Apendicular de la Progenie de Ratones CF-1

作者：Pablo Lizana; Ricardo Henriquez; Pablo Muoz

来源：International Journal of Morphology, 2012.

• Estres prenatal
• Raton CF-1
• Alizarina roja
• Esqueleto apendicular
• Morfometria
• Restriccion de movimiento
• Prenatal Stress
• CF1 Mouse
• Alizarin red
• Appendicular Skeletal
• Morphometric
• Movement restriction

Prenatal stress has been associatedwith alterationsin weight andbody size, as well as disturbances inthe process ofdevelopingskeletalossification, occurringduring childbirth and the early stagesof life.However, theeffectevidence of prenatal stresson bone growthand development duringthe gestation periodhas been low; therefore, it is unknown whether these alterations are associated with potential for growth disorders Because of this, thestudy aims todetermine theshort-term effectsof prenatal stress onthe CF-1mouse bone structure growth inyour date of birth. The female mice were divided randomly in two groups: controlled (n=2) and stressed (n=3). The latter was put under stress by means of movement restriction during the last week of gestation. Second, an evaluation of their gestational development was made, obtaining measurements of their weight. Finally, diaphanization with KOH and staining with Alizarin red was used to measure the length of their appendicular bones and their flat pelvic bones, of 53 P0 mice (25 control; 28 stressed during gestation). The stressed mice%26apos;s body weight (p=0.02) and the length of their appendicular bones (radii, p=0.0011; ulnae, p= 0.0001; humeri, p=0.0001; femorae, p=0.0006; tibiae, p=0.0015) were affected significantly in contrast with the controlled group. On the other hand,there were nosignificant differences inmaternal bodyweightand length ofthe mice pelvic bones (isquium, ilium; p%26gt;0.05). The prenatal stress by means of movement restriction alters the osseous appendicular morphology of the CF-1 mouse evaluated at birth. El estr谷s prenatal se ha asociado con alteraciones en el peso y el tama o corporal, as赤 como trastornos en el proceso osificaci車n en el esqueleto en desarrollo, que se producen durante el parto y las primeras etapas de vida. Sin embargo, la evidencia de los efectos del estr谷s prenatal sobre el crecimiento de los huesos y el desarrollo durante el per赤odo de gestaci車n ha sido baja, desconoci谷ndose si estas alteraciones est芍n relacionadas con trastornos del crecimiento. El objetivo fue determinar los efectos a corto plazo del estr谷s prenatal en la estructura 車sea del rat車n CF-1 en el d赤a de nacimiento. Las hembras gestantes fueron divididas aleatoriamente en dos grupos: control (n=2) y estresado (n=3). Este 迆ltimo fue puesto bajo estr谷s por restricci車n de movimiento durante la 迆ltima semana de gestaci車n. Evaluando el peso corporal en la progenie al nacer (grupo control n=25; grupo estresado n=28), para posteriormente diafanizar mediante KOH y te ir con alizarina roja, midiendo la longitud de huesos largos a