Cloning and bioinformatic analysis of β-AS gene from Panax japonicus [竹节参β-香树素合成酶基因克隆及生物信息学分析]

作者：Yuan D.; Jiao M.-T.; Xiu C.; Zhang C.-C.; Liu Z.-Q.; He Y.-M.; Yuan C.-F.

来源：Chinese Traditional and Herbal Drugs, 2020, 51(19): 5028-5034.

DOI：10.7501/j.issn.0253-2670.2020.19.022

• Bioinformatics analysis
• Gene cloning
• Panax japonicus
• Triterpene saponin
• Β-amyrin synthase gene

Objective: The key enzyme of triterpene saponin metabolism was cloned and its sequence, structure and function were analyzed by bioinformatics. Methods: RNAs were extracted from the leaves of Panax japonicus The full-length cDNA sequences of β-AS were cloned by utilizing RT-PCR method, and the sequence was connected to the pMDTM18-T for cloning and sequencing.β-AS protein characteristics in transplanted species and cultivated species of P. japonicus were predicted and compared by bioinformatics analysis and the phylogenetic tree of β-AS protein was constructed. Results: The β-AS sequences in transplanted species and cultivated species of P. japonicus were obtained, which had 2 286 bp ORF and encoded 761 amino acids. There were little differences between the two varieties of β-AS proteins in physicochemical properties, secondary structure, tertiary structure, and phosphorylation sites, which may lead to show difference in catalytic activity. Conclusion: This work also obtained the full-length of cDNA sequence of β-AS gene in transplanted and cultivated varieties of P. japonicus, and provided a systemic sequence analysis of β-AS proteins, which can provide the useful information for β-AS studies in the future.

• 单位
  三峡大学