胸腺素α_1的DIC固相化学合成与鉴定

作者：甘一如; 黄永东; 刘晋生; 张雪竹; 张贵峰

来源：化工学报, 2004, (02): 305-308.

• 胸腺素α1
• 固相多肽合成
• 二异丙基碳二亚胺(DIC)
• 反相高效液相色谱法
• 聚丙烯酰胺凝胶电泳(SDSPAGE)法
• 液相色谱/质谱(LC/MS) thymosin α 1
• solid-phase peptide synthesis
• N
• N′-diisopropylcarbodiimide (DIC)
• reversed-phase high performance liquid chromatography
• SDS-polyacrylamide gel electrophoresis
• liquid chromatography/mass spectroscopy(LC/MS)

Thymosin α 1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α 1 was synthesized with the solid-phase method, by using the base-liable Fmoc group to protect the α-amino aci...

• 单位
  中国科学院过程工程研究所; 生化工程国家重点实验室; 天津大学