锌指蛋白217基因表达与人卵巢癌细胞的关系(英文)

作者：Lilin Hang; Min Zhang; Fanliang Meng; Mei Zhong; Jing Li

来源：The Chinese-German Journal of Clinical Oncology, 2014, 13(11): 539-544.

• ovarian cancer
• zinc-finger protein 217(ZNF217) gene
• gene expression
• proliferation
• invasion
• tumor metastasis

Objective: The aim of the study was to investigate the correlation of zinc-finger protein 217(ZNF217) gene expression with the biological behavior of human ovarian cancer HO-8910 cells. Methods: The expression of ZNF217 in ovarian carcinoma cell lines was detected by RT-PCR and Western blot, respectively. The biological behaviors of the transfectants were investigated by MTT, in vitro Boyden chamber and in vivo invasion assay, respectively. Results: RT-PCR and Western blotting revealed that transfection of ZNF217 into the HO-8910 cells significantly increased their proliferation along with markedly enhanced in vitro and in vivo invasion and metastatic abilities. MTT assay showed that the proliferation ability of p EGFPN1-ZNF217/HO-8910 cells was significantly higher than that of p EGFP-N1/HO-8910 cells and HO-8910 cells(P < 0.001). The Boyden chamber assay showed that the numbers of migrating p EGFP-N1-ZNF217/HO-8910, p EGFP-N1/ HO-8910 and HO-8910 cells were(141.25 ± 13.91) cells /200 × field,(82.50 ± 11.73) cells /200 × field and(81.75 ± 12.12) cells /200 × field, respectively, with a significant difference between them(F = 29.274, P < 0.001). The nude mouse experiment showed that the in vivo tumor formation ability of p EGFP-N1-ZNF217/HO-8910 cells was significantly higher than that of p EGFP-N1/HO-8910 cells(P < 0.001). Conclusion: Based on these clinical and laboratory observations, we conclude that ZNF217 may contribute to ovarian cancer invasion and metastasis, and associated with worse clinical outcomes. We evaluated ZNF217’s role as a biomarker of ovarian carcinogenesis and tumor progression in patient samples and explored possible molecular mechanisms in promoting tumor growth and invasion.