Determination of the amphetamine-like designer drugs methcathinone and 4-methylmethcathinone in urine by LC-MS/MS D谷termination par LC-MS/MS des ※drogues de synth豕se§: la m谷thcathinone et la 4-m谷thylm谷thcathinone (substances analogues aux amph谷tamines) dans les urines

作者：Grueninger Dirk; Englert Ralf

来源：Annales de Toxicologie Analytique, 2011.

DOI：10.1051/ata/2011008

• Beta-keto amphetamines
• designer drugs
• liquid chromatography-tandem mass spectrometry
• methcathinone
• ephedrone
• 4-methylmethcathinone
• mephedrone
• Beta-keto-amphetamines
• drogues de synthese
• tandem chromatographie liquide/spectrometrie de masse
• methcathinone
• ephedrone
• 4-methylmethcathinone
• mephedrone

Purpose: Beta-keto amphetamines are a new generation of designer drugs which have gained popularity among abusers in the last decade. A sensitive and reliable method for the diagnostics of individuals who abuse these substances is very important, especially in the case of overdose or concomitant use during a drug substitution therapy. Methods: We developed a quantitative HPLC-tandem mass spectrometry method for the determination of methcathinone and 4-methylmethcathinone in urine, based on an easy liquid-liquid extraction technique coupled to fast reversed phase chromatography. Results: For both analytes we obtained a linearity range from 0.5 to at least 2000 ng/mL. In different urinary matrices the accuracy for 4-methylmethcathinone was within 9% and for methcathinone within 17% recovery of the target concentration. The repeatability and the intermediate precision of 50 different test series were between 2 and 14%. The limit of quantification was established to be 2 ng/mL for methcathione and 0.8 ng/mL for 4-methylmethcathinone. Conclusions: The described HPLC-tandem mass spectrometry method is reliable and robust and has the advantage of accurate selectivity, avoiding wrong positive results. It is convenient for clinical laboratories involved in the control of drug substitution therapy or the confirmation of drug consumption. Objectif : Les beta-k谷to-amph谷tamines sont une nouvelle g谷n谷ration de drogues de synth豕se dont l＊usage s＊est r谷pandu chez les toxicomanes au cours des dix derni豕res ann谷es. Une m谷thode pr谷cise et fiable de d谷pistage chez les personnes susceptibles d＊utiliser ces substances est importante, dans le cas de surdose ou d＊utilisation concomitante pendant un traitement de substitution. M谷thodes : Nous avons d谷velopp谷 une m谷thode par CLHP coupl谷e 角 la spectrom谷trie de masse en tandem pour le dosage de la m谷thcathinone et de la 4-m谷thylm谷thcathinone dans l＊urine, qui repose sur une technique d＊extraction liquide-liquide simple coupl谷e 角 une chromatographie rapide 角 phase inverse. R谷sultats : Pour les deux substances 角 doser la m谷thode est lin谷aire de 0,5 角 au moins 2000 ng/mL. Dans diff谷rentes matrices urinaires, la pr谷cision pour la 4-m谷thylm谷thcathinone 谷tait d＊environ 9 %, et pour la m谷thcathinone d＊environ 17 % de r谷cup谷ration de concentration cible. La r谷p谷tabilit谷 et la pr谷cision interm谷diaire sur 50 dosages diff谷rents s＊谷chelonnaient entre 2 et 14 %. La limite de quantification s＊est 谷tablie 角 2 ng/mL pour la m谷thcathinone et 角 0,8 ng/mL pour la 4-m谷thylm谷thcathinone. Conclusions : Cette m谷thode de dosage de la m谷thcathinone et de