Recombinase‐mediated cassette exchange ( RMCE ) for monoclonal antibody expression in the commercially relevant CHOK1SV cell line

作者：Lin Zhang; Mara C Inniss; Shu Han; Mark Moffat; Heather Jones; Baohong Zhang; Wendy L Cox; James R Rance; Robert J Young

来源：Biotechnology Progress, 2015, 31(6): 1645-1656.

DOI：10.1002/btpr.2175

• RMCE
• monoclonal antibody
• site specific integration
• stability
• cell line development

To meet product quality and cost parameters for therapeutic monoclonal antibody (mAb) production, cell lines are required to have excellent growth, stability, and productivity characteristics. In particular, cell line generation stability is critical to the success of a program, especially where hig...