Fluid shear stress induces Runx-2 expression via upregulation of PIEZO1 in MC3T3-E1 cells

作者：Song, Jidong; Liu, Liying; Lv, Leifeng; Hu, Shugang; Tariq, Alkhatatbeh; Wang, Wei^*; Dang, Xiaoqian^*

来源：Cell Biology International, 2020, 44(7): 1491-1502.

DOI：10.1002/cbin.11344

• fluid shear stress
• osteogenesis
• PIEZO1

Mechanically induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 is a newly discovered mechanically activated ion channel in bone cells. This study aimed to explore the involvement of PIEZO1 in mechanical loading (fluid shear stress)-induced signaling cascades that control osteogenesis. The results showed that fluid shear stress increased PIEZO1 expression in MC3T3-E1 cells. The fluid shear stress elicited the key osteoblastic gene Runx-2 expression; however, PIEZO1 silencing using small interference RNA blocked these effects. The AKT/GSK-3 beta/beta-catenin pathway was activated in this process. PIEZO1 silencing impaired mechanically induced activation of the AKT/GSK-3 beta/beta-catenin pathway. Therefore, the results demonstrated that MC3T3-E1 osteoblasts required PIEZO1 to adapt to the external mechanical fluid shear stress, thereby inducing osteoblastic Runx-2 gene expression, partly through the AKT/GSK-3 beta/beta-catenin pathway.

• 单位
  西安交通大学